[Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions]
1Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Objective:
To analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomics.
Methods:
Two-dimensional electrophoresis(2-DE) was performed to obtain the 2-D gel electrophoresis patterns of dental pulp. Mass spectrometry (MS) was used to analyze several different selected spots in the expression proteins.
Results:
No significant difference in protein expression was found between normal and moderate carious dental. Two protein spots were absent in heat-damaged group and 8 spots showed significantly down-regulated. Seven proteins were identified by MS.
Conclusions:
In the present study, no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues. However, down-regulation of pulp protein in thermal stimulation was observed.
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