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Lectin-modified trifunctional nanobiosensors for mapping cell surface glycoconjugates.

Min Xie¹, Jun Hu, Yan-Min Long

¹College of Chemistry and Molecular Sciences and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China.

Biosensors & Bioelectronics

|September 16, 2008

Summary

Researchers developed novel fluorescent-magnetic nanobiosensors by embedding quantum dots and magnetic nanoparticles. These trifunctional nanobiosensors can detect and isolate A549 cancer cells by targeting specific cell surface sugars.

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Area of Science:

Biotechnology and Nanomedicine
Materials Science
Cell Biology

Background:

Nanomaterial-based nanobiosensors are gaining importance for biological applications.
Specific cell surface glycoconjugates can serve as biomarkers for diseases like cancer.

Purpose of the Study:

To fabricate fluorescent-magnetic trifunctional nanobiosensors (TFNS) for detecting and isolating A549 cells.
To investigate the quantitative and qualitative analysis of cell surface glycoconjugates using lectin-modified TFNS.

Main Methods:

Co-embedding quantum dots and gamma-Fe(2)O(3) magnetic nanoparticles into poly(styrene/acrylamide) nanospheres.
Modifying nanospheres with wheat germ agglutinin (WGA), peanut agglutinin (PNA), or Dolichos biflorus agglutinin (DBA) to create lectin-TFNS.

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Utilizing fluorescence intensity and flow cytometry to analyze glycoconjugate binding and cell capture.

Main Results:

Successfully produced fluorescent-magnetic-biotargeting trifunctional nanobiosensors.
Demonstrated that lectin-TFNS can quantify N-acetylglucosamine, d-galactosamine, and N-acetylgalactosamine on A549 cells.
Showcased the ability of WGA-modified TFNS to capture A549 cells (0.5mg captured 7.0 x 10^4 cells).

Conclusions:

Lectin-modified TFNS are effective tools for mapping cell surface glycoconjugates.
These nanobiosensors can be utilized for the recognition and isolation of targeted cells, such as A549 cancer cells.