Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Soft Colloidal Robots: Magnetically Guided Liquid Crystal Torons for Targeted Micro-Cargo Delivery.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Genetic engineering of gut commensals: heterologous protein expression in <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> CECT8145.

Current research in microbial sciences·2026
Same author

Topological defects lead to energy transfer in active nematics.

Nature communications·2026
Same author

Active nematic pumps.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

E. coli filament buckling modulates Min patterning and cell division.

Nature communications·2025
Same author

Physical communication pathways in bacteria: an extra layer to quorum sensing.

Biophysical reviews·2025

Related Experiment Video

Updated: Dec 13, 2025

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.0K

A spatial toggle switch drives boundary formation in development.

Oriol Canela-Xandri1, Francesc Sagués, Ramón Reigada

  • 1Computer Simulation and Modeling Lab, and Institut de Química Teórica i Computacional IQTCUB, Parc Científic de Barcelona, Barcelona, Spain.

Biophysical Journal
|September 16, 2008
PubMed
Summary
This summary is machine-generated.

We discovered a multicellular network motif acting as a spatial toggle switch, crucial for understanding developmental boundary formation. Simultaneous characterization of protein expression and activity is essential for mechanism elucidation.

More Related Videos

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions
07:09

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions

Published on: May 2, 2019

6.4K
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.9K

Related Experiment Videos

Last Updated: Dec 13, 2025

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.0K
Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions
07:09

Integrating Visual Psychophysical Assays within a Y-Maze to Isolate the Role that Visual Features Play in Navigational Decisions

Published on: May 2, 2019

6.4K
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

6.9K

Area of Science:

  • Developmental biology
  • Systems biology
  • Cellular network dynamics

Background:

  • Boundary formation is a fundamental process in development.
  • Understanding the molecular mechanisms driving boundary formation is crucial.

Purpose of the Study:

  • To introduce a multicellular network motif that functions as a spatial toggle switch.
  • To elucidate how developmental boundaries are formed.
  • To emphasize the need for simultaneous characterization of protein expression and activity.

Main Methods:

  • In silico modeling and simulation of multicellular networks.
  • Analysis of protein expression and activity patterns.
  • In vivo experimental validation.
  • Parameter estimation and robustness analysis.

Main Results:

  • A novel multicellular network motif acting as a spatial toggle switch was identified.
  • The study demonstrates the necessity of simultaneously analyzing protein expression and activity for understanding boundary formation.
  • In silico experiments validated the model across different genetic backgrounds, aligning with in vivo observations.

Conclusions:

  • The identified network motif provides a mechanistic explanation for spatial toggle switch behavior in development.
  • Simultaneous characterization of protein dynamics is key to deciphering complex biological mechanisms.
  • The findings offer insights into the robustness and parameter dependencies of boundary formation processes.