[Long-term culture and a morphological study of endothelial cells from human aorta]

Endothelial cells from human aorta were successfully cultured first in this Lab in our country. Cells survived and passed through 10-15 generations. Long term cultured endothelial cells from human aorta were observed under phase-contrast microscope, scanning, transmission electron microscope, and investigated immunocytochemically by immunofluorescence of specific antibody against Factor-VIII related surface antigen, and ABC method using monoclonal antibody 9B9 against human angiotensin-converting enzyme. Medium RPMI-1640 supplemented with 20% human serum, endothelial cell growth factor 200 micrograms/ml, heparin 100 micrograms/ml and gelatin coated flasks were very important conditions for long term culture of human endothelial cell.