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Development and validation of a fast quantitative method for plasma dimethylarginines analysis using liquid
Oceania D'Apolito1, Giuseppe Paglia, Filomena Tricarico
1Clinical Biochemistry, Department of Biomedical Sciences, Faculty of Medicine-University of Foggia, Viale L. Pinto, 1-71100 Foggia, Italy.
Objectives:
The aim of this work was to implement a fast, accurate and simple method to quantify plasma ADMA and SDMA, in a run time suitable for routine analysis.
Design And Methods:
We developed and validated a hydrophilic interaction chromatographic method coupled to tandem mass spectrometry (HILIC-MS/MS) for separation and simultaneous quantification of Arginine (Arg) and its dimethylarginines, ADMA and SDMA, with a short run time (less than 5 min) using a small volume of human plasma (0.02 mL).
Results:
Correlation coefficients (r) of the calibration curves ranged from 0.9926 to 0.9984. Within-day and between-day imprecision (CV%) and inaccuracy (%), carry-over and recovery were also evaluated for validation. Preliminary data of Arg, ADMA and SDMA from 30 apparently healthy subjects and type 2 diabetic patients (n=33) with and without kidney dysfunction were calculated and some statistical differences occurred among them (p<0.05).
Conclusions:
Data from calibration curves and quality controls reveal that the method is accurate and precise. Healthy subjects and diabetic patients' values are in agreement with those reported in other studies.
