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Multi-spectral angular domain optical imaging in biological tissues using diode laser sources.

Fartash Vasefi1, Bozena Kaminska, Paulman K Y Chan

  • 1Simon Fraser University, School of Engineering Science, 8888 University Drive, Burnaby, BC, Canada. fartash@ieee.org

Optics Express
|September 17, 2008
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Summary

Angular Domain Imaging (ADI) uses a special filter to detect unscattered light. This study shows ADI works with multiple laser wavelengths, achieving high resolution in biological tissue.

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Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Photonics

Background:

  • Scattered photons in biological tissues limit imaging resolution.
  • Angular Domain Imaging (ADI) offers a method to reject scattered light.
  • Multi-wavelength imaging can provide complementary information.

Purpose of the Study:

  • To construct and evaluate an Angular Domain Imaging system using diode lasers.
  • To demonstrate the feasibility of multi-wavelength ADI.
  • To assess the imaging performance of ADI in biological tissue.

Main Methods:

  • Construction of an ADI system with diode lasers at red and near-infrared wavelengths.
  • Testing ADI performance with varying thicknesses of chicken breast tissue.
  • Utilizing a micromachined angular filter to differentiate scattered and non-scattered photons.

Main Results:

  • Successful implementation of a multi-wavelength ADI system.
  • Demonstration of ADI's capability to image through scattering biological media.
  • Achieved a spatial resolution of less than 0.5 mm in 5 mm thick chicken breast using a 975 nm laser.

Conclusions:

  • Multi-wavelength Angular Domain Imaging is feasible for biological tissue imaging.
  • ADI demonstrates potential for high-resolution imaging in scattering media.
  • The developed ADI system shows promise for biomedical applications.