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Sense and antisense OsDof12 transcripts in rice.

Dejun Li1, Chunhua Yang, Xiaobing Li

  • 1State Key Laboratory of Plant Genomics & National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, PR China. djli@genetics.ac.cn

BMC Molecular Biology
|September 18, 2008
PubMed
Summary
This summary is machine-generated.

Sense and antisense transcripts of the OsDof12 gene in rice are co-expressed due to shared promoter elements. Their expression is influenced by developmental stages, drought, and darkness, suggesting coordinated regulation.

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Area of Science:

  • Plant Molecular Biology
  • Gene Expression Regulation
  • Rice Genetics

Background:

  • Antisense transcription is a common biological process in plants and mammals.
  • Previous microarray data indicated co-expression of sense (OsDof12) and antisense (OsDof12os) transcripts in rice leaves.

Purpose of the Study:

  • To analyze the detailed expression patterns of OsDof12 and OsDof12os transcripts in rice.
  • To investigate the potential mechanisms underlying their co-expression.

Main Methods:

  • Detailed expression pattern analysis of OsDof12 and OsDof12os transcripts across different rice tissues and developmental stages.
  • Analysis of gene expression under environmental stresses (drought and dark treatment).
  • Utilized ProOsDof12-GUS and ProOsDof12os-GUS transgenic rice plants to track expression patterns.
  • Bioinformatic analysis of cis-regulatory elements in the promoters of OsDof12 and OsDof12os.

Main Results:

  • OsDof12 and OsDof12os transcripts were co-expressed in the same tissues and showed reciprocal expression patterns during different developmental stages.
  • Expression of both transcripts was induced by drought stress and inhibited by dark treatment.
  • GUS expression patterns in transgenic plants mirrored the endogenous transcript expression.
  • Analysis revealed significant overlap (53 out of 74 classes) in predicted cis-regulatory elements between the two promoters.

Conclusions:

  • The co-expression of OsDof12 and OsDof12os is likely due to shared cis-regulatory elements and common promoter characteristics.
  • These findings suggest a coordinated regulatory mechanism for sense and antisense transcription at the OsDof12 locus in rice.