Related Experiment Video
Updated: Jun 30, 2026
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
Published on: June 16, 2019
Synthesis of 3,5-difluorotyrosine-containing peptides: application in substrate profiling of protein tyrosine
Bhaskar Gopishetty1, Lige Ren, Tiffany M Waller
1Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.
Abstract:
Fully protected 3,5-difluorotyrosine (F2Y), Fmoc-F2Y(tBu)-OH, is efficiently prepared by a chemoenzymatic process and incorporated into individual peptides and combinatorial peptide libraries. The F2Y-containing peptides display kinetic properties toward protein tyrosine phosphatases (PTPs) similar to their corresponding tyrosine-containing counterparts but are resistant to tyrosinase action. These properties make F2Y a useful tyrosine surrogate during peptide library screening for optimal PTP substrates.
