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Updated: Jun 30, 2026
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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
Published on: August 24, 2022
Large-scale gene trapping in C57BL/6N mouse embryonic stem cells
Gwenn M Hansen1, Diane C Markesich, Michael B Burnett
1Lexicon Pharmaceuticals Incorporated, The Woodlands, Texas 77381, USA. ghansen@lexpharma.com
Genome Research
|September 19, 2008
Summary
Researchers created a large mouse gene trap mutant resource with over 350,000 embryonic stem (ES) cell clones. These clones enable germline transmission and knockout mouse generation, aiding genetic research.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Developmental Biology
Background:
- The development of comprehensive genetic resources is crucial for understanding gene function.
- Gene trap technology offers a powerful method for generating mutations in embryonic stem cells.
Purpose of the Study:
- To report the creation and characterization of a large-scale mouse gene trap mutant resource.
- To demonstrate the utility of this resource for generating knockout mice and studying gene function.
Main Methods:
- Construction of a mouse gene trap mutant resource in the C57BL/6N background.
- Generation of over 350,000 sequence-tagged embryonic stem (ES) cell clones.
- Analysis of insertion sites, mutagenicity prediction, and germline transmission capability.
Main Results:
- A resource of >350,000 sequence-tagged ES cell clones was established.
- Mutations were identified in over 10,000 genes, with a bias towards first intron insertions.
- Successful germline transmission and generation of knockout mice were demonstrated.
Conclusions:
- The developed gene trap resource provides a valuable tool for the scientific community.
- This resource facilitates the study of gene function through knockout mouse models.
- The sequence-tagged nature allows for precise genetic analysis and genotyping.
