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Updated: Jun 30, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
Effective mutagenesis of Vibrio fischeri by using hyperactive mini-Tn5 derivatives
Noreen L Lyell1, Anne K Dunn, Jeffrey L Bose
1Department of Microbiology, University of Georgia, 1000 Cedar Street, Athens, GA 30602, USA.
Abstract:
We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of approximately 10(-6). This simple and inexpensive technique represents a significant improvement over previous methods for transposon mutagenesis of V. fischeri and should be applicable to many other bacteria.
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