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Updated: Jun 30, 2026
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Expression and polimorphism of IFN-gamma gene in patients with cervical cancer
M K Kordi Tamandani1, R C Sobti, M Shekari
1Department of Biotechnology, Panjab University, Chandigrah, India. dor_kordi@yahoo.com
Background:
Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-gamma ) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity.
Aim:
To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-gamma messenger RNA (mRNA) at the tumor site.
Methods:
DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-gamma gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-gamma mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay.
Results:
It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI - 2.05-5.2, P
Conclusion:
This is the first study to provide an evidence for effecting of IFN-gamma gene on the risk of cervical cancer in north Indian population.
