Jove
Visualize
Contact Us

Related Experiment Videos

Agar disk diffusion susceptibility tests with cilofungin (LY 121019).

A Espinel-Ingroff1, S Shadomy, S White

  • 1Department of Internal Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0038.

Journal of Medical and Veterinary Mycology : Bi-Monthly Publication of the International Society for Human and Animal Mycology
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Etest ECVs/ECOFFs for Detection of Resistance in Prevalent and Three Nonprevalent <i>Candida</i> spp. to Triazoles and Amphotericin B and Aspergillus spp. to Caspofungin: Further Assessment of Modal Variability.

Antimicrobial agents and chemotherapy·2021
Same author

Synergising tools for capacity assessment and One Health operationalisation.

Revue scientifique et technique (International Office of Epizootics)·2019
Same author

Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in <i>Candida</i> and <i>Aspergillus</i> Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods.

Antimicrobial agents and chemotherapy·2018
Same author

Posaconazole MIC Distributions for Aspergillus fumigatus Species Complex by Four Methods: Impact of <i>cyp51A</i> Mutations on Estimation of Epidemiological Cutoff Values.

Antimicrobial agents and chemotherapy·2018
Same author

Multicenter, International Study of MIC/MEC Distributions for Definition of Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular Methods.

Antimicrobial agents and chemotherapy·2017
Same author

Multicenter Study of Method-Dependent Epidemiological Cutoff Values for Detection of Resistance in Candida spp. and Aspergillus spp. to Amphotericin B and Echinocandins for the Etest Agar Diffusion Method.

Antimicrobial agents and chemotherapy·2016
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Experimental cilofungin disks effectively predict yeast susceptibility. Both 10- and 20-microgram disks showed strong correlation with minimal inhibitory concentration (MIC) values, aiding in antifungal treatment decisions.

Area of Science:

  • Mycology
  • Antimicrobial Susceptibility Testing

Background:

  • Cilofungin (LY 121019) is a lipopeptide antifungal agent with demonstrated in vitro and in vivo activity against Candida species.
  • Accurate prediction of antifungal susceptibility is crucial for effective treatment of yeast infections.

Purpose of the Study:

  • To evaluate the utility of experimental 10- and 20-microgram cilofungin disks for predicting in vitro susceptibility.
  • To establish a correlation between disk diffusion test results and minimal inhibitory concentration (MIC) values for cilofungin.

Main Methods:

  • Fifty-two pathogenic yeast isolates, including Candida albicans, Candida tropicalis, and Candida glabrata, were tested.
  • Agar dilution MIC tests and agar disk diffusion tests were performed using standardized culture conditions (yeast nitrogen base with asparagine and dextrose, 35°C, 24h incubation).

Related Experiment Videos

Main Results:

  • Regression analysis revealed a high degree of correlation between MIC values and disk inhibition zones.
  • The 10-microgram disk showed a correlation coefficient (r) of 0.9278, and the 20-microgram disk showed an r value of 0.9082.

Conclusions:

  • Both 10- and 20-microgram cilofungin disks are reliable for predicting probable in vitro minimal inhibitory concentrations.
  • These disk diffusion methods offer a practical approach for assessing cilofungin susceptibility in clinical laboratories.