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Updated: Jun 30, 2026
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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
Published on: June 28, 2016
Plasmon-controlled fluorescence: a new paradigm in fluorescence spectroscopy
Joseph R Lakowicz1, Krishanu Ray, Mustafa Chowdhury
1University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 W. Lombard St., Baltimore, MD 21201, USA.
The Analyst
|September 24, 2008
Summary
Metallic surfaces dramatically alter fluorescence spectroscopy by enabling near-field interactions. This review explores plasmonics and metal-fluorophore interactions for novel bioassays and devices.
Area of Science:
- Biophysics
- Spectroscopy
- Nanotechnology
Background:
- Fluorescence spectroscopy is a cornerstone of biological research, traditionally relying on far-field optical energy detection.
- Far-field detection involves measuring emitted light propagating away from the fluorophore at macroscopic distances.
- Recent advancements focus on the interactions between fluorophores and metallic nanostructures.
Purpose of the Study:
- To provide an intuitive explanation of plasmonics and near-field interactions.
- To review recent research on metal-fluorophore interactions.
- To explore the potential of these interactions for new experimental techniques, probes, bioassays, and devices.
Main Methods:
- This review synthesizes existing literature on plasmonics and near-field optical phenomena.
- It focuses on the theoretical underpinnings of metal-fluorophore interactions.
- The review summarizes experimental findings and applications.
Main Results:
- Near-field interactions, occurring within a wavelength of the fluorophore, significantly alter spectral properties.
- These interactions are mediated by the electron clouds of metallic surfaces or particles.
- Altered emission properties differ markedly from classical fluorescence observations.
Conclusions:
- Metal-fluorophore interactions, driven by plasmonics, offer novel ways to manipulate fluorescence.
- These phenomena pave the way for advanced bioassays, sensing devices, and experimental methodologies.
- Understanding near-field effects is crucial for future innovations in fluorescence-based research.
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