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Related Concept Videos

Xylem and Transpiration-driven Transport of Resources02:03

Xylem and Transpiration-driven Transport of Resources

The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Jun 30, 2026

Processing the Loblolly Pine PtGen2 cDNA Microarray
07:01

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Published on: March 20, 2009

Identification of conserved core xylem gene sets: conifer cDNA microarray development, transcript profiling and

Nathalie Pavy1, Brian Boyle, Colleen Nelson

  • 1Centre d'Etude de la Forêt, 1030 rue de la Médecine, Université Laval, Québec, Québec, Canada, G1K 7P4.

The New Phytologist
|September 25, 2008
PubMed
Summary
This summary is machine-generated.

Researchers investigated wood formation by comparing white spruce genes to model plants. They identified 360 xylem-preferential genes, with 31 conserved across species, advancing our understanding of tree biology.

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Area of Science:

  • Plant molecular biology
  • Forestry science
  • Comparative genomics

Background:

  • Wood formation is a complex process crucial for trees.
  • Comparative transcriptomics aids in understanding conserved molecular mechanisms across species.
  • White spruce (Picea glauca) is a key conifer species for forestry and ecological studies.

Purpose of the Study:

  • To investigate the molecular basis of wood formation in white spruce using a custom cDNA microarray.
  • To compare gene expression profiles between white spruce xylem and other tissues (phloem, needles).
  • To identify conserved genes and novel genes involved in xylem differentiation by comparing with model plants like Arabidopsis.

Main Methods:

  • Development of a white spruce cDNA microarray with 10,400 sequences.
  • Transcript profiling of secondary xylem, phloem, and needles.
  • Computational analysis to identify differentially expressed sequences and conserved transcripts.
  • Comparison of white spruce gene sets with core xylem gene sets from Arabidopsis.

Main Results:

  • Identification of 360 xylem-preferential gene sequences in white spruce.
  • Functional annotations of differentially expressed genes were consistent with angiosperm and herbaceous plant studies.
  • Discovery of 31 transcripts highly conserved in both sequence and xylem expression between gymnosperms (spruce) and angiosperms.
  • Identification of novel genes potentially involved in xylem differentiation, including TUBBY-like domain proteins (TLPs) and a gibberellin insensitive (gai) gene.

Conclusions:

  • The study provides a valuable resource for understanding conifer wood formation at the molecular level.
  • Comparative analysis highlights conserved genetic pathways in xylem development across diverse plant lineages.
  • Novel genes identified offer new avenues for research into xylem differentiation and tree growth regulation.