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GATA4 is essential for jejunal function in mice.

Michele A Battle1, Benjamin J Bondow, Moriah A Iverson

  • 1Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA.

Gastroenterology
|September 25, 2008
PubMed
Summary
This summary is machine-generated.

The zinc-finger transcription factor GATA4 is crucial for jejunal function, controlling fat and cholesterol absorption. Its absence alters jejunal gene expression, impacting intestinal identity.

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Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Genetics

Background:

  • The zinc-finger transcription factor GATA4 is known to regulate jejunal gene expression.
  • Its specific role in controlling jejunal physiology remains largely uncharacterized.

Purpose of the Study:

  • To investigate the contribution of GATA4 in regulating jejunal physiology.
  • To determine the impact of GATA4 deletion on intestinal fat and cholesterol absorption and gene expression.

Main Methods:

  • Generation of mice with specific Gata4 gene deletion in the small intestinal epithelium.
  • Measurement of plasma cholesterol, phospholipids, and intestinal absorption of dietary fat and cholesterol.
  • Global gene expression profiling using gene array analysis.

Main Results:

  • Mice lacking GATA4 in the intestine exhibited a significant impairment in cholesterol and dietary fat absorption.
  • GATA4-null jejunum lost expression of 53% of jejunal-specific genes and gained 47% of ileal-specific genes.
  • Decreased expression of lipid/cholesterol transporter mRNAs and increased expression of bile acid absorption protein mRNAs were observed.

Conclusions:

  • GATA4 is essential for normal jejunal function, including fat and cholesterol absorption.
  • GATA4 plays a critical role in establishing and maintaining jejunal identity versus ileal identity.