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Related Concept Videos

Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Lipids as Anchors01:32

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...

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Related Experiment Video

Updated: Jun 30, 2026

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Long-term storable and shippable lipid bilayer membrane platform.

Tae-Joon Jeon1, Jason L Poulos, Jacob J Schmidt

  • 1University of California, Los Angeles, Department of Bioengineering, Los Angeles, CA 90095, USA.

Lab on a Chip
|September 25, 2008
PubMed
Summary

This study introduces a novel method for creating stable lipid bilayer membranes using high freezing-point mixtures. These robust precursors can be stored and later reassembled into functional membranes for practical ion channel measurements.

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Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
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Area of Science:

  • Biophysics
  • Materials Science

Background:

  • Conventional lipid bilayer membranes are fragile and have short lifetimes, requiring immediate use upon preparation.
  • This limits their widespread application and storage potential.

Purpose of the Study:

  • To develop a method for creating stable, storable lipid bilayer membrane precursors.
  • To enable the development of practical and disposable platforms for ion channel measurements.

Main Methods:

  • Utilized high freezing-point lipid-solvent mixtures to reversibly arrest lipid bilayer self-assembly.
  • Developed a solid-form bilayer precursor that can be stored and shipped.
  • Demonstrated resumption of bilayer self-assembly upon thawing.

Main Results:

  • The solid-form bilayer precursor is robust and can be stored indefinitely.
  • Upon thawing, the precursor self-assembles into biologically functional membranes.
  • Integration with inexpensive chips yields a compact, practical, and disposable platform for ion channel measurements.

Conclusions:

  • A novel method for creating stable and storable lipid bilayer membrane precursors has been established.
  • This advancement facilitates the development of robust and disposable biosensing platforms.
  • The technology holds significant potential for advancing ion channel research and diagnostics.