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Related Experiment Video

Updated: Jun 30, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Proteomic dissection of viral pathogenesis.

H C Liu, J Hicks, D Yoo

Developments in Biologicals

|September 27, 2008

Summary

Proteomics, the study of proteins, helps scientists understand viral pathogenesis. This research explores various proteomic techniques to uncover how viruses cause disease, aiding vaccine and treatment development.

Area of Science:

Virology
Proteomics
Molecular Biology

Background:

Viral pathogenesis is complex and understanding it is crucial for developing effective vaccines and treatments.
Proteomics offers advanced methods to study the roles of proteins in viral disease mechanisms.

Purpose of the Study:

To review and highlight various proteomic approaches used to dissect viral pathogenesis.
To emphasize the importance of proteomics in understanding virus-host interactions and disease progression.

Main Methods:

Mass spectrometry (MS)-based techniques, including MALDI-MS and MALDI-TOF MS, for analyzing viral antigens and host proteins.
Liquid chromatography coupled with MS/MS for identifying proteins in specific cellular compartments, such as lipid droplets.
Yeast two-hybrid systems used in conjunction with MS to identify viral-host interactions and potential receptors.

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Last Updated: Jun 30, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Main Results:

Proteomic studies have identified specific proteins involved in viral pathogenesis, such as those contributing to Hepatitis C virus carcinogenicity.
High-throughput MS approaches have been successfully applied to study viral antigenicity, exemplified by influenza virus research.
Proteomics, combined with other methods, reveals complex interactions between viral and host proteins, offering insights into viral mechanisms.

Conclusions:

Proteomic strategies are powerful tools for elucidating the intricate mechanisms of viral pathogenesis.
Continued application of diverse proteomic techniques will accelerate the development of novel antiviral therapies and vaccines.
Understanding protein-level interactions is key to combating viral diseases effectively.