Related Experiment Video
Updated: Jun 30, 2026

Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep
Published on: January 19, 2014
Single nucleotide polymorphism (SNP) in prion protein gene (PRNP) exon-3 in Gaddi sheep
Abstract:
Nine PCR-SSCP conformations in PRNPthe exon-3 genomic DNA segment covering codons 136, 154 and 171 were observed. DNA sequences revealed 9 unique variants (DQ885789-DQ885796). At codon 171 of exon-3, two alleles, Q and R, had a frequency of 0.336 and 0.664, respectively. Genotypes QQ, QR and RR were observed in 25.86, 15.51 and 58.62% of animals, respectively, showing deviation from Hardy-Weinberg equilibrium. At codon 154, all genotypes were RR. At codon 136 AA, AV and W genotypes were 86.21, 10.34 and 3.44%, respectively, and frequencies of the alanine (A) and valine (V) alleles were 0.914 and 0.086, respectively, where the W genotype had lower frequency than expected. Polymorphisms were also observed at codons 137, 143 and 173 of exon-3.
More Related Videos
11:41Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
Published on: March 10, 2015
09:34Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Published on: April 4, 2018
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
Comparing Copy Number Variations and SNPs
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Leaky Scanning