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Related Concept Videos

Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jun 30, 2026

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

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Published on: August 20, 2019

Mapping the BDA20 gene to BTAX.

N B Stafuzza, S Kata, J E Womack

    Developments in Biologicals
    |September 27, 2008
    PubMed
    Summary

    The bovine BDA20 gene, a major cow dander allergen, was successfully mapped to the bovine X chromosome (BTAX). This finding aids in understanding occupational allergies in dairy farmers.

    Area of Science:

    • Immunology
    • Genetics
    • Veterinary Science

    Background:

    • The lipocalin family includes diverse proteins with significant functional variation.
    • Bovine BDA20 is a major cow dander allergen, causing occupational asthma in dairy farmers.
    • Understanding the genetic basis of allergens is crucial for managing allergic diseases.

    Purpose of the Study:

    • To determine the chromosomal location of the bovine BDA20 gene.
    • To investigate the genetic linkage of BDA20 within the bovine genome.

    Main Methods:

    • Somatic cell panel analysis was used to assign the BDA20 gene.
    • Radiation hybrid (RH) mapping was employed to confirm gene localization.
    • Two-point LOD score analysis was performed to assess genetic linkage.

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    Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

    Published on: August 15, 2019

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    Last Updated: Jun 30, 2026

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    06:41

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    Associated Chromosome Trap for Identifying Long-range DNA Interactions
    14:49

    Associated Chromosome Trap for Identifying Long-range DNA Interactions

    Published on: April 23, 2011

    Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
    09:37

    Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

    Published on: August 15, 2019

    Main Results:

    • The BDA20 gene was assigned to the bovine X chromosome (BTAX) with 97% concordance to marker MAF45.
    • RH mapping confirmed the BTAX localization of BDA20.
    • Significant linkage was found between BDA20 and XBM451 (LOD score 22.1).

    Conclusions:

    • The bovine BDA20 gene is located on the X chromosome.
    • This genetic mapping provides a foundation for further research into cow dander allergy mechanisms.
    • Identifying the chromosomal location of allergens like BDA20 can inform strategies for managing occupational allergies.