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Mass propagation of Rauwolfia serpentina L. Benth
U Salma1, M S M Rahman, S Islam
1Department of RDDR, Modem Herbal Group, Dhaka 1217, Bangladesh.
Abstract:
A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L(-1) BA and 0.2 mg L(-1) NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L(-1) each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival.
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