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CroFab reconstitution in various media: an in vitro solubility study
Rais Vohra1, Rick Clark, Michael Kelner
1David Geffen School of Medicine at UCLA, Emergency Medicine, Olive View Medical Center, Sylmar, CA, USA. raisvohra@hotmail.com
Clinical Toxicology (Philadelphia, Pa.)
|September 30, 2008
Summary
Crotalidae Polyvalent Ovine Immune Fab (CroFab) antivenom showed slightly better solubility in half-normal saline. No significant protein loss occurred after refrigeration or passage through IV tubing.
Area of Science:
- Pharmacology
- Biochemistry
Background:
- Investigated Crotalidae Polyvalent Ovine Immune Fab (CroFab) antivenom solubility in non-FDA approved solutions.
- Assessed potential adsorption of CroFab to plastic intravenous (IV) tubing.
Purpose of the Study:
- To determine the solubility of CroFab in solutions beyond those listed in its FDA-approved insert.
- To evaluate the stability and potential loss of CroFab when administered through plastic IV tubing.
Main Methods:
- Expired CroFab vials were reconstituted with normal saline, lactated Ringer's solution, or half-normal saline.
- Protein concentration was measured post-reconstitution, after 4-hour refrigeration, and after passage through IV tubing.
Main Results:
- Half-normal saline resulted in higher measured protein yields compared to normal saline.
- Lactated Ringer's solution showed higher protein concentrations than normal saline at one measurement point.
- No adsorption of CroFab to plastic IV tubing was observed.
Conclusions:
- CroFab exhibits slightly increased solubility in hypotonic solutions like half-normal saline.
- Antivenom integrity was maintained after 4-hour refrigeration and passage through plastic IV tubing.
- Findings are relevant for clinicians and pharmacists using non-standard reconstitution solutions for CroFab.
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