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Related Experiment Video

Updated: Jun 30, 2026

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

High-throughput Agrobacterium-mediated barley transformation.

Joanne G Bartlett¹, Sílvia C Alves, Mark Smedley

¹Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK. wendy.harwood@bbsrc.ac.uk.

|September 30, 2008

Summary

A new barley transformation protocol significantly improves efficiency to 25%. This robust method simplifies genetic modification for barley (Hordeum vulgare) and related crops, aiding functional genomics research.

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Area of Science:

Plant Science
Genetics
Agricultural Science

Background:

Barley (Hordeum vulgare) is a globally important cereal crop.
It serves as a model organism for wheat research.
Existing barley transformation methods often lack efficiency and simplicity.

Purpose of the Study:

To develop a robust, simple, and reproducible protocol for barley transformation.
To achieve higher transformation efficiencies than currently available methods.

Main Methods:

Immature barley embryos were infected with Agrobacterium strain AGL1.
Vectors from the pBract series containing the hygromycin resistance (hpt) gene were used.
The firefly luciferase (luc) gene was employed as a reporter for large-scale experiments.

Main Results:

The developed protocol achieved average transformation efficiencies of 25%.
Hundreds of independent transgenic barley lines were produced.
A significant proportion of these lines contained single copies of the reporter gene.

Conclusions:

The new protocol offers substantial improvements in efficiency and ease of use for barley transformation.
This advancement facilitates the development of functional genomics resources in barley.
The method holds potential for direct crop improvement applications.