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Related Concept Videos

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

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Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
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Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

Published on: November 11, 2020

Collagen and myosin characterization by orientation field second harmonic microscopy.

Christophe Odin1, Thomas Guilbert, Alia Alkilani

  • 1Institut of Physics of Rennes IPR/UMR CNRS 6251, University of Rennes I, Campus deBeaulieu, Bat 11A, 35042 Rennes Cedex, France. christophe.odin@univ-rennes1.fr

Optics Express
|October 1, 2008
PubMed
Summary

This study introduces Orientation Field-Second Harmonic Microscopy (OF-SHM) to map collagen and myosin fibril orientation. This new method uses Second Harmonic Generation (SHG) imaging to reveal fibril structures in biological systems.

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Last Updated: Jun 30, 2026

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
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Published on: November 11, 2020

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy
08:49

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Area of Science:

  • Biophysics
  • Microscopy
  • Biomaterials

Background:

  • Collagen and myosin fibrils are key biological structures.
  • Second Harmonic Generation (SHG) is a nonlinear optical phenomenon.
  • Existing methods for fibril orientation mapping have limitations.

Purpose of the Study:

  • To develop a novel microscopy technique for determining fibril orientation.
  • To quantify the nonlinear susceptibility tensor components for collagen and myosin.
  • To establish a correlation between fibril orientation and the nonlinear susceptibility tensor.

Main Methods:

  • Utilizing four polarization Second Harmonic Generation (SHG) images from a scanning microscope.
  • Calculating the orientation of the principal axis of the nonlinear susceptibility tensor chi(2) per pixel.
  • Determining the ratio rho = chi33/chi15 of the principal components of chi(2).

Main Results:

  • Successfully determined the orientation of the principal axis of chi(2) for each pixel.
  • Obtained the ratio rho for collagen (1.6-1.8) and myosin (0.5-0.6).
  • Demonstrated that the orientation of the principal axis of chi(2) correlates with fibril orientation.

Conclusions:

  • Orientation Field-Second Harmonic Microscopy (OF-SHM) is a straightforward method for reconstructing fibril orientation fields.
  • OF-SHM is applicable to various biological systems, from sarcomeres to whole embryos.
  • This technique offers a new way to study fibril organization at different scales.