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Better reporting is better science: Community-defined minimal reporting requirements for light microscopy.

Paula Montero Llopis1, Chloë van Oostende-Triplet2, Nathalie Gaudreault3

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Microscopy reporting guidelines are needed to improve research transparency and data reuse. This study introduces a minimal checklist for clear, actionable guidance to enhance community standards in microscopy.

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Area of Science:

  • Microscopy and Imaging Science
  • Scientific Methodology
  • Data Reproducibility

Background:

  • Incomplete reporting of microscopy methods hinders scientific transparency, reproducibility, and data reuse.
  • Current reporting guidelines for microscopy are insufficient, lacking broad endorsement and accessibility.
  • There is a critical need for standardized, easy-to-implement reporting standards in microscopy.

Purpose of the Study:

  • To present a minimal set of essential microscopy reporting requirements.
  • To develop a checklist that integrates human- and machine-readable formats.
  • To provide clear, actionable guidance for researchers, reviewers, and publishers to improve microscopy reporting.

Main Methods:

  • Development of a bare minimal microscopy reporting requirements checklist.
  • Integration of human-readable and machine-readable components into the checklist.
  • Focus on essential elements for transparent and reproducible microscopy methods reporting.

Main Results:

  • A concise checklist of essential microscopy reporting requirements has been established.
  • The checklist is designed for both human interpretation and machine processing.
  • The proposed requirements aim to enhance clarity and actionability in microscopy methods reporting.

Conclusions:

  • Implementing this minimal checklist can significantly improve the transparency and reproducibility of microscopy research.
  • Standardized reporting will facilitate data reuse and advance community standards in the field.
  • This initiative provides a practical tool to address the persistent challenge of incomplete microscopy methods reporting.