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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 29, 2026

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

Published on: March 11, 2014

Automated voltage-clamp technique.

Andrea Ghetti¹, António Guia, Jia Xu

¹AVIVA Biosciences Corp., San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 2, 2008

Summary

Automated planar patch-clamp systems, like PatchXpress/Sealchip, enhance ion channel function measurement. These platforms overcome limitations of traditional voltage-clamp electrophysiology, enabling high-throughput, standardized drug discovery experiments.

Area of Science:

Electrophysiology
Pharmacology
Biophysics

Background:

Traditional voltage-clamp electrophysiology is crucial for ion channel function analysis but suffers from low throughput and complexity.
Limitations in throughput, skill requirements, and standardization hinder its application in pharmaceutical drug discovery.

Purpose of the Study:

To provide an overview of novel planar patch-clamping platforms for automated electrophysiology.
To describe the experimental workflow on the PatchXpress/Sealchip automated system for ion channel quantification.

Main Methods:

Utilizes new electrophysiology platforms based on planar electrodes for automated cell-to-electrode sealing.
Incorporates computer-controlled fluidics, voltage-clamping electronics, and automated data handling for parallel experiments.

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Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism

Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism

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Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Published on: May 5, 2018

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Last Updated: Jun 29, 2026

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

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Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism

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Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Published on: May 5, 2018

Main Results:

New planar electrode geometry facilitates automation, increasing throughput and reducing the skill needed.
Automated systems enable high-consistency, parallel electrophysiology experiments in a fully automated mode.

Conclusions:

Automated planar patch-clamping systems, exemplified by PatchXpress/Sealchip, are emerging as the new standard for ion channel function quantification.
These platforms significantly improve the efficiency and accessibility of electrophysiology for drug discovery.