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Updated: Jun 29, 2026

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
Published on: August 9, 2017
Electrophysiological properties of embryonic stem cells during differentiation into cardiomyocyte-like cell types
Antoni C G van Ginneken1, Arnoud C Fijnvandraat
1Department of Experimental Cardiology and Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, The Netherlands.
Abstract:
The method described here to differentiate mouse embryonic stem (ES) cells into cardiomyocytes is adapted from Maltsev et al. and results in a high percentage of spontaneously beating cardiomyocyte-like cells. In order to determine to what extent the differentiating ES cells resemble true cardiomyocytes, the cells were electrophysiologically characterized during differentiation, using the whole-cell variant of the patch-clamp technique. Action potentials (APs) and membrane currents were recorded and analyzed off-line to determine electrophysiological changes during development.
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