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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Related Experiment Video

Updated: Jun 29, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

NRED: a database of long noncoding RNA expression.

Marcel E Dinger1, Ken C Pang, Tim R Mercer

  • 1ARC Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia.

Nucleic Acids Research
|October 3, 2008
PubMed
Summary
This summary is machine-generated.

A new database, NRED, offers gene expression data for thousands of long non-protein-coding RNAs (ncRNAs) in humans and mice. This resource aids the study of these important molecules.

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Last Updated: Jun 29, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

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Published on: March 1, 2019

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Thousands of long non-protein-coding RNAs (>200 nt) have been identified in mammals.
  • The biological roles and functions of most long non-protein-coding RNAs are currently unknown.
  • A comprehensive resource is needed to facilitate the study of long non-protein-coding RNAs.

Purpose of the Study:

  • To create a public repository, the Noncoding RNA Expression Database (NRED), for long non-protein-coding RNA gene expression data.
  • To provide gene expression information for thousands of long non-protein-coding RNAs in human and mouse.
  • To offer ancillary data and facilitate research into long non-protein-coding RNA function.

Main Methods:

  • Compilation of gene expression data from microarray and in situ hybridization experiments.
  • Integration of diverse datasets including evolutionary conservation, secondary structure, genomic context, and antisense relationships.
  • Development of a web interface for advanced searching and data downloading.

Main Results:

  • The Noncoding RNA Expression Database (NRED) is now publicly available.
  • NRED contains gene expression data for numerous long non-protein-coding RNAs in human and mouse.
  • The database includes comprehensive ancillary information for featured long non-protein-coding RNAs.

Conclusions:

  • NRED serves as a valuable resource for the scientific community.
  • The database will significantly advance the study and understanding of long non-protein-coding RNAs.
  • NRED facilitates research into the biological significance and function of long non-protein-coding RNAs.