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Updated: Jun 29, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
A novel thermophilic lysozyme from bacteriophage phiIN93
Isao Matsushita1, Hideshi Yanase
1Energy Technology Laboratories, Osaka gas Co, Ltd, Torishima, Osaka, Japan. imatsu@osakagas.co.jp
Abstract:
The lysozyme of bacteriophage phiIN93 was purified to apparent homogeneity with Carboxymethyl Sepharose and Hydroxyapatie columns from lysates of the phage grown on Thermus aquaticus TZ2. The enzyme is a single polypeptide chain with a molecular weight of 33,000. From the determined N-terminal amio acids of the enzyme, the locus of the gene was specified on a phiIN93 genome. The enzyme was not similar to egg white lysozyme, T4 phage lysozyme, or lambda phage lysozyme. The enzyme, phiIN93 lysozyme, was found to be a novel type of thermophilic lysozyme, which lyses specifically Thermus sp. cells, and exhibited conspicuous thermal stability at 95 degrees C for 1h in the presence of beta-mercaptoethanol.
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