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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jun 29, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Rapid screening assay for KRAS mutations by the modified smart amplification process.

Kenji Tatsumi¹, Yasumasa Mitani, Jun Watanabe

¹Genome Exploration Research Group (Genome Network Project Core Group), RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, Japan. landy-tk@bd5.so-net.ne.jp

The Journal of Molecular Diagnostics : JMD

|October 4, 2008

Summary

A new PNA-clamp SMAP-2 assay rapidly detects KRAS mutations in cancer specimens within 60 minutes. This method offers a simple, sensitive, and accurate approach for clinical identification of critical cancer mutations.

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Published on: September 25, 2018

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

Area of Science:

Molecular Biology
Oncology

Background:

The smart amplification process version 2 (SMAP-2) assay enables precise detection of somatic mutations.
Existing methods for mutation detection can be time-consuming and complex for routine clinical use.

Purpose of the Study:

To develop a rapid and practical SMAP-2 assay for detecting cancer and metastasis.
To create a modified SMAP-2 assay capable of detecting any single codon change.

Main Methods:

Development of modified SMAP-2 assays for single codon mutation detection.
Design of primers for KRAS codon 12 amplification and use of peptide nucleic acid (PNA) clamp for wild-type allele discrimination.
Implementation of the PNA-clamp SMAP-2 assay for rapid mutation detection.

Main Results:

The PNA-clamp SMAP-2 assay detected KRAS mutations within 60 minutes, including sample preparation.
The assay demonstrated perfect concordance with polymerase chain reaction-restriction fragment length polymorphism and direct sequencing in pancreatic cancer patient samples.
The method successfully identified all mutant KRAS alleles while excluding the wild-type sequence.

Conclusions:

The PNA-clamp SMAP-2 method is a rapid, simple, and highly sensitive assay for detecting cancer mutations.
This assay is suitable for routine clinical identification of critical amino acid substitutions, such as KRAS codon 12 mutations.
The developed assay facilitates faster and more efficient cancer diagnosis and metastasis detection.