Related Experiment Video
Updated: Jun 29, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Effect of double-application or the application of a hydrophobic layer for improved efficacy of one-step self-etch
Maurício Albuquerque1, Mariana Pegoraro, Gracielle Mattei
1School of Dentistry, University of Oeste de Santa Catarina, Joaçaba, SC, Brazil.
Objectives:
To evaluate the use of two alternative modes of application (double-application and placement of a hydrophobic resin coat) compared with the manufacturers' directions on the microtensile bond strength (microTBS) of one-step, self-etch systems to enamel and dentin.
Materials And Methods:
Resin composite buildups were bonded to the buccal and lingual ground enamel surfaces and occlusal dentin of third molars using the following adhesives: Xeno III (XE), GBond (GB), Adper Prompt L-Pop (AD) and Clearfil SE Bond (CSE) as the control. The adhesive systems were applied: (1) following the manufacturer's directions (MD), (2) with double-application (DA) or with (3) additional placement of a hydrophobic resin layer (HR) after following the manufacturer's directions. After storage in water (24 hours/37 degrees C), the bonded specimens were sectioned into sticks (0.8 mm2) that were tested until failure at a crosshead speed of 0.5 mm/minute. Data from each substrate were subjected to two-way ANOVA and Tukey's test (alpha = 0.05).
Results:
The interaction Adhesive vs. Application mode was statistically significant (p < 0.05) for both substrates. In dentin, XE and GB achieved high microTBS in the HR group (p < 0.05). For AD, the highest microTBS were observed in the DA group (p < 0.05). For enamel, similar microTBS was observed for XE and GB among the three modes of application, while the highest pTBS of AD was observed in the HR group.
Conclusion:
The effects of using the double-application and placement of a hydrophobic resin coat on microtensile dentin-bond strength values seem to be effective; however, these techniques in enamel were adhesive-dependent.
Related Concept Videos
The Electrical Double Layer
Masking and Demasking Agents
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on the metal...
Leveling Effect
Preparation of Samples for Electron Microscopy
