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Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

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Updated: Jun 29, 2026

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

Single-scan 2D DOSY NMR spectroscopy.

Yoav Shrot1, Lucio Frydman

  • 1Department of Chemical Physics, Weizmann Institute of Science, 76100 Rehovot, Israel. Yoav.shrot@weizmann.ac.il

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|October 7, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces an ultrafast, single-scan 2D DOSY NMR method using spatial encoding. This technique efficiently measures molecular displacements, offering advantages over existing methods for rapid diffusion measurements.

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physical Chemistry
  • Analytical Chemistry

Background:

  • Multidimensional NMR spectra are typically acquired over multiple scans.
  • Spatial encoding allows for multidimensional NMR data acquisition in a single scan.
  • Diffusion Ordered SpectroscopY (DOSY) is crucial for analyzing molecular diffusion.

Purpose of the Study:

  • To explore the application of spatial encoding for acquiring 2D DOSY spectra in a single scan.
  • To introduce and evaluate an "ultrafast" modality for single-scan 2D DOSY.
  • To demonstrate the advantages and limitations of this novel approach.

Main Methods:

  • Implementation of spatial encoding techniques for NMR.
  • Development of novel pulse sequences for single-scan 2D DOSY.
  • Acquisition and analysis of 2D DOSY spectra for various samples.

Main Results:

  • The developed method enables 2D DOSY spectra acquisition within a single scan.
  • The "ultrafast" modality shows similarities to existing single-scan methods but offers distinct advantages.
  • Demonstrated utility across diverse sample types.

Conclusions:

  • Spatial encoding provides an effective strategy for ultrafast, single-scan 2D DOSY NMR.
  • This methodology enhances efficiency in measuring molecular displacements.
  • Potential for further extensions and applications in NMR spectroscopy.