Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual Acidic pH-Responsive Post-Crosslinked E-Spun Nanofibrous Scaffolds Exhibiting Enhanced Disassembly and Release for Localized Cancer Treatment.

Advanced healthcare materials·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

High-throughput screening of per- and polyfluoroalkyl substances in human plasma using biocompatible solid-phase microextraction coupled with mass spectrometry via microfluidic open interface.

Analytica chimica acta·2026
Same author

Comprehensive analysis of exhaled breath VOCs using GC-MS and GCĂ—GC-TOF-MS: a comparative platform evaluation with TFME and NTD sampling for free and total concentrations.

Analytical and bioanalytical chemistry·2025
Same author

Preoperative untargeted SPME-LC/HRMS-based metabolomic profiling in cardiac surgical patients identified prognostic biomarkers for postoperative outcomes.

Analytica chimica acta·2025
Same journal

Whole-body mass spectrometry imaging reveals metabolome and lipid peroxidation heterogeneity in zebrafish xenografts of esophageal squamous cell carcinoma.

Analytical and bioanalytical chemistry·2026
Same journal

A robust and validated method for the determination of 21 urinary metabolites of 15 plasticizers, including phthalates, DEHTP, and DINCH, by online SPE and liquid chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry·2026
Same journal

A label-free membrane-based biosensor array with AuNP-modified PDMS for sensitive and specific detection of alpha-fetoprotein.

Analytical and bioanalytical chemistry·2026
Same journal

Smartphone-integrated one-step colorimetric glucose detection at physiological pH enabled by a haloperoxidase mimic.

Analytical and bioanalytical chemistry·2026
Same journal

Chemiluminescence functionalized magnetic nanoparticles-based biosensor for sensitive detection of glucose, uric acid, and cholesterol.

Analytical and bioanalytical chemistry·2026
Same journal

Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.

Analytical and bioanalytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Recent developments in solid-phase microextraction.

Sanja Risticevic1, Vadoud H Niri, Dajana Vuckovic

  • 1Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.

Analytical and Bioanalytical Chemistry
|October 7, 2008
PubMed
Summary
This summary is machine-generated.

Recent advancements in solid-phase microextraction (SPME) technology enhance high-throughput analysis in food, environmental, and bioanalytical chemistry. New automated systems and SPME devices significantly improve sample preparation efficiency and performance.

More Related Videos

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

Related Experiment Videos

Last Updated: Jun 29, 2026

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
07:26

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

Published on: January 1, 2016

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Sample Preparation Technologies

Background:

  • Solid-phase microextraction (SPME) is a versatile technique for sample preparation.
  • Traditional SPME methods face limitations in throughput and automation.
  • Continuous innovation is driven by the need for faster and more efficient analysis in various fields.

Purpose of the Study:

  • To review recent developments in SPME technology.
  • To highlight advancements in automation, high-throughput analysis, and new SPME device designs.
  • To discuss applications in food, environmental, and bioanalytical chemistry.

Main Methods:

  • Review of recent literature on SPME advancements.
  • Focus on automation in Gas Chromatography (GC) and Liquid Chromatography (LC) applications.
  • Analysis of new SPME device designs, including needle trap, thin-film microextraction, and cold-fibre headspace SPME.

Main Results:

  • SPME automation has enabled high-throughput applications for both GC and LC.
  • New autosamplers and metal fibre assemblies increase sample throughput for SPME-GC.
  • A multi-SPME autosampler allows parallel preparation of up to 96 samples for LC, achieving >1,000 samples/day.
  • Novel SPME devices offer improved performance over conventional fibre arrangements.

Conclusions:

  • Recent developments in SPME technology have significantly enhanced analytical efficiency.
  • Automation and new device designs are crucial for high-throughput analysis in demanding applications.
  • SPME continues to evolve as a powerful tool in food, environmental, and bioanalytical chemistry.