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Updated: Jun 29, 2026

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
[Study on alkaloids in Lobelia sessilifolia by ESI-MS(n)]
Xiu-Li Wang1, Jia-Ming Sun, Yang Song
1Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese Medicine, Changchun 130117, China.
Objective:
To make qualitative analysis on alkaliod in Lobelia sessilifolia.
Method:
The ESI-MS(n) method was used for qualitative analysis.
Result:
The structures of two alkaliods in plant extract of L. sessilifolia were successfully analyzed and identified as norlobelanine and 8, 10-diethyllobelidione by electrospray ionization multistage mass spectrometry in positive mode. Then the fragmentation mechanisms were explored.
Conclusion:
SPE and ESI-MS(n) could simply and rapidly provide qualitative examination of alkaliods in L. sessilifolia.

