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Related Experiment Video

Updated: Jun 29, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

A functional nitric oxide reductase model.

James P Collman¹, Ying Yang, Abhishek Dey

¹Department of Chemistry, Stanford University, Stanford, CA 94305, USA. jpc@stanford.edu

Proceedings of the National Academy of Sciences of the United States of America

|October 8, 2008

Summary

This summary is machine-generated.

A novel heme/nonheme model effectively reduces nitric oxide (NO) to nitrous oxide (N2O) using a "trans" mechanism. This study clarifies bacterial nitric oxide reductase function.

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Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Area of Science:

Bioinorganic Chemistry
Enzyme Mechanisms
Coordination Chemistry

Background:

Nitric oxide reductases (NORs) are crucial enzymes in microbial denitrification.
Understanding NOR mechanisms is vital for developing novel bioremediation and industrial catalysts.
Existing models often lack the complexity to fully replicate bacterial NOR activity.

Purpose of the Study:

To present a functional heme/nonheme diiron model for nitric oxide reductase (NOR).
To elucidate the reaction mechanism of NO reduction by this model compound.
To investigate the role of individual iron centers in the catalytic cycle.

Main Methods:

Synthesis and characterization of a novel diiron compound with heme and nonheme iron centers.
Reaction of the reduced diiron model with nitric oxide (NO).
Spectroscopic analysis to identify intermediates and products.

Main Results:

The fully reduced diiron model reacts with two equivalents of NO.
Formation of one equivalent of nitrous oxide (N2O) and a bis-ferric product.
NO binds to both heme Fe and nonheme Fe, forming individual ferrous nitrosyl species.
A mixed-valence species (oxidized heme, reduced nonheme Fe(B)) showed no NO reduction activity.

Conclusions:

The observed NO reduction pathway supports a
trans
]
The heme and nonheme iron centers play distinct, sequential roles in NO reduction.
This model provides key insights into the catalytic mechanism of bacterial NORs.