Thin frozen film method for visualization of storage proteins in mature rice grains
Yuhi Saito1, Nobuaki Nakatsuka, Takanari Shigemitsu
1Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Bioscience, Biotechnology, and Biochemistry
|October 8, 2008
Abstract:
There are technical difficulties in obtaining intact sections of cereal grains in which mature cells and their subcellular structures are well preserved. Here we describe a simple method for sectioning hard mature rice grains. It makes possible accurate localization of storage proteins in high-quality histological sections of rice endosperm.
