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Real-time PCR assays for high-throughput human platelet antigen typing

Simon E McBride1

  • 1Department of Platelet Immunology, NHS Blood and Transplant, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2008
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...