Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proposed Development of a New Staging System for Hearing Loss: Countermeasure 2 of the Hearing Health Collaborative.

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·2025
Same author

Techniques in Management of Cholesteatoma: Subtotal Petrosectomy; Blind Sac Closure.

Otolaryngologic clinics of North America·2024
Same author

Otoferlin gene therapy restores hearing in deaf children.

Molecular therapy : the journal of the American Society of Gene Therapy·2024
Same author

Debilitating Gentamicin Ototoxicity: Case Report and Recommendations Against Routine Use in Surgical Prophylaxis.

The Annals of otology, rhinology, and laryngology·2023
Same author

Rare Coding Variants in Patients with Non-Syndromic Vestibular Dysfunction.

Genes·2023
Same author

Novel candidate genes for cholesteatoma in chronic otitis media.

Frontiers in genetics·2023

Related Experiment Video

Updated: Jun 29, 2026

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
22:02

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Published on: June 30, 2012

Electroporation-mediated gene transfer to the developing mouse inner ear.

John V Brigande1, Samuel P Gubbels, David W Woessner

  • 1Oregon Hearing Research Center, Oregon Health & Science University, Portland OR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 8, 2008
PubMed
Summary

A new method allows researchers to study mammalian inner ear development by directly manipulating genes within the otic vesicle. This technique overcomes in utero accessibility challenges, aiding the understanding of inner ear formation and function.

More Related Videos

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
09:03

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

Published on: January 12, 2015

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
03:52

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

Published on: March 2, 2018

Related Experiment Videos

Last Updated: Jun 29, 2026

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
22:02

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Published on: June 30, 2012

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
09:03

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

Published on: January 12, 2015

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
03:52

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse

Published on: March 2, 2018

Area of Science:

  • Developmental biology
  • Genetics
  • Otolaryngology

Background:

  • The mammalian inner ear develops from the otic placode, forming the otic vesicle (otocyst).
  • Progenitor cells within the otocyst give rise to inner ear sensory and non-sensory cells.
  • Studying early in utero development is challenging due to inaccessibility.

Purpose of the Study:

  • To present a novel experimental embryological method for gene misexpression in the developing mammalian inner ear.
  • To enable functional studies of genes regulating inner ear development.

Main Methods:

  • Microinjection of expression plasmids into the otic vesicle lumen through the uterine wall.
  • Electroporation of plasmids into otic epithelial progenitor cells.
  • Downstream analysis of transfected embryonic or postnatal inner ear tissue.

Main Results:

  • Successfully established a method for targeted gene misexpression in the developing otic vesicle.
  • Demonstrated feasibility for investigating gene function in inner ear development.

Conclusions:

  • This technique provides a valuable tool for dissecting the molecular mechanisms of mammalian inner ear formation.
  • Facilitates research into congenital hearing loss and inner ear disorders.