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Updated: Jun 29, 2026
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Published on: January 16, 2017
In vivo verification of protein interactions in the inner ear by coimmunoprecipitation
Margaret C Harvey1, Bernd H A Sokolowski
1Department of Otolaryngology-HNS, University of South Florida, College of Medicine, Tampa, FL, USA.
Abstract:
Genomics has provided us with vast amounts of data and thus, the challenge to identify and characterize gene products. Proteomics analysis, using methods such as yeast two-hybrid screenings, isoelectric focusing, and mass spectroscopy, generate potentially useful information. To determine functional relationships between and among proteins, however, the initial data for putative protein interactions must first be validated. One technique, which is considered the gold standard, is coimmunoprecipitation.
