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Updated: Jun 29, 2026
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
[The auditory study of guinea pig immunized with P0 protein]
Qing Cheng1, Xi Lin, Jibao Wang
1Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. cqjj74@163.com
Objective:
To investigate the auditory characteristics of guinea pigs immunized with purified P0 protein from inner ear of guinea pigs.
Method:
Purified inner ear P0 protein was made by Preparative SDS-PAGE. To evaluate the results of Auditory Brainstem Response (ABR), Compound Action Potention (CAP), and Distortion Product Oto-acoustic Emissions (DPOAE) in guinea pigs immunized with purified guinea pigs inner ear myelin protein P0.
Result:
Seven ears of the guinea pigs immunized with purified inner ear P0 protein developed hearing loss. In the ABR study, peak latencies of wave I, III and the interpeak latency of I -III, I-IV were elevated in this group of guinea pigs compared with the control group (P < 0.01). But the interpeak latency of III-IV did not change. In the CAP study, the threshold elevated and latency prolonged (P < 0.01). No significant change of DPOAE was found in the P0-sensitized guinea pigs when compared to controls (P > 0.05). Even though the contra-lateral suppressive effect had the depressed tendency, but there was no significant different when compared with the controls (P > 0.05).
Conclusion:
Purified inner ear P0 protein is an important autoimmune inner ear antigen and can develop autoimmune disease of the auditory nerve.
