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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jun 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Playing tag with quantitative proteomics.

Anton Iliuk¹, Jacob Galan, W Andy Tao

¹Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.

Analytical and Bioanalytical Chemistry

|October 10, 2008

Summary

This summary is machine-generated.

Quantitative proteomics utilizes stable isotope labeling for precise cellular analysis. This review highlights chemical labeling techniques, including soluble polymer-based isotopic labeling (SoPIL), for dynamic biological studies.

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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

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Last Updated: Jun 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Area of Science:

Proteomics
Biochemistry
Cellular Biology

Background:

Quantitative proteomics is crucial for understanding dynamic cellular processes.
Stable isotope labeling offers powerful methods for protein quantitation.
Existing techniques include metabolic labeling and chemical labeling post-protein extraction.

Purpose of the Study:

To review current quantitative proteomics techniques.
To focus on chemical labeling strategies using stable isotopes.
To introduce and evaluate soluble polymer-based isotopic labeling (SoPIL).

Main Methods:

Review of literature on stable isotope labeling in proteomics.
Detailed examination of chemical labeling approaches.
Evaluation of soluble polymer-based isotopic labeling (SoPIL).

Main Results:

Stable isotope labeling provides essential quantitative data for cellular processes.
Chemical labeling, particularly with SoPIL, offers efficient and homogeneous labeling.
Selection of appropriate quantitation methods is critical for experimental success.

Conclusions:

Stable isotope labeling is a vital tool in quantitative proteomics.
SoPIL represents a promising advancement in chemical labeling techniques.
Careful experimental design is necessary for effective application of these methods.