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Published on: May 3, 2017
[Study on glutamate substrate-induced regulation on expression of glutamate transporter GLAST of mouse retinal Müller
Xiao-bo Xia1, Xia Zhou, Qi Zeng
1Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China. xbxia@yahoo.com
Objective:
To study glutamate substrate-induced regulation on the expression of glutamate transporter L-glutamate/L-aspartate transporter (GLAST) of retinal Müller cells (RMCs) in mice.
Methods:
Activity of GLAST was measured through L-3 H-glutamate uptake detection. The expression of GLAST protein in lysates cells was studied using Western blot.
Results:
Glutamate was applied to cultured RMCs at various concentrations for 30 min. L-3 H-glutamate accumulation in RMCs increased as the increase of concentration of substrate glutamate and reached the maximum at 100 micromol/L glutamate. Glutamate treatment of RMCs increased GLAST transporter expression as compared with untreated cultures. Glutamate at 100 micromol/L caused a maximal increase of GLAST protein expression.
Conclusion:
Glutamate substrate increases GLAST activity and up-regulate the expression of GLAST protein.
