Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunogenicity and safety of AS03-adjuvanted A/Astrakhan/3212/2020 (H5N8)-like influenza vaccine in adults: Phase 1/2, observer-blinded, randomized trial.

Human vaccines & immunotherapeutics·2026
Same author

Relationship between Central Corneal Thickness and Endothelial Cell Density in Individuals with Primary Glaucoma and the General Population Living at High Altitudes.

Journal of current ophthalmology·2026
Same author

Machine learning-based prediction of soiling losses in photovoltaic modules under different cleaning frequencies: an experimental investigation.

Scientific reports·2026
Same author

Comparison of ocular biometry and refraction measurements among myopic children using myopia master with IOL master 700 and NIDEK ARK-500 autorefractor.

Indian journal of ophthalmology·2026
Same author

Multi-class classification of brain tumors using optimized CNN and transfer learning techniques.

Scientific reports·2026
Same author

Evaluation of Inflammatory Cytokines in Tears of Individuals with Dry Eye Related to Prolonged Computer Use.

Journal of current ophthalmology·2026

Related Experiment Video

Updated: Jun 29, 2026

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

Self-assembled monolayer for low density lipoprotein detection.

Zimple Matharu1, Sunil K Arya, G Sumana

  • 1Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, New Delhi-110012, India.

Journal of Molecular Recognition : JMR
|October 11, 2008
PubMed
Summary
This summary is machine-generated.

This study developed a new biosensor for detecting low-density lipoprotein (LDL) using immobilized heparin on a gold surface. The sensor demonstrates sensitivity for LDL detection within a specific concentration range.

More Related Videos

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

Related Experiment Videos

Last Updated: Jun 29, 2026

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Low-density lipoprotein (LDL) detection is crucial for cardiovascular disease risk assessment.
  • Current methods for LDL detection can be complex and time-consuming.
  • Development of sensitive and rapid biosensors is needed for improved diagnostics.

Purpose of the Study:

  • To develop a novel biosensor for the sensitive detection of low-density lipoprotein (LDL).
  • To immobilize heparin (HEP) onto a gold (Au) surface via a 4-aminothiophenol (ATP) self-assembled monolayer (SAM).
  • To characterize the performance of the developed biosensor using electrochemical and optical techniques.

Main Methods:

  • Covalent immobilization of heparin onto 4-aminothiophenol self-assembled monolayer on gold-coated glass.
  • Characterization using cyclic voltammetry (CV) and scanning electron microscopy (SEM).
  • Detection and kinetic analysis using surface plasmon resonance (SPR) measurements.

Main Results:

  • The fabricated HEP/ATP/Au electrode showed sensitivity to LDL detection.
  • Successful LDL detection was achieved in the concentration range of 0.03 µM (10 mg/dL) to 0.39 µM (130 mg/dL).
  • Association (k(a) = 9.67 x 10^1 M⁻¹s⁻¹) and dissociation (k(d) = 2.64 x 10⁻⁴ s⁻¹) rate constants were determined.

Conclusions:

  • The developed heparin-based biosensor offers a sensitive platform for LDL detection.
  • The immobilization strategy and characterization methods provide a foundation for further biosensor development.
  • This approach holds potential for improved cardiovascular risk monitoring.