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Updated: Jun 29, 2026
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
Published on: January 10, 2017
Improved Detection Limit and Stability of Amperometric Carbon Nanotube-Based Immunosensors by Crosslinking Antibodies
Vito Cataldo1, Abhay Vaze, James F Rusling
1Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060, USA.
Abstract:
Amperometric immunosensor configurations featuring covalently bound anti-biotin antibodies (Ab) embedded into a polylysine (PLL)-single walled carbon nanotube (SWCNT) composite layer were evaluated. Assemblies were made by first oxidizing pyrolytic graphite (PG) electrodes to form surface carboxylic acid groups, to which PLL, SWCNTs and anti-biotin were covalently linked. Incorporating SWCNT into PLL-antibody assemblies improved the amperometric detection limit for biotin (Ag) labeled with horseradish peroxidase to 10 fmol mL(-1). Anti-biotin embedded into the PLL matrix had improved thermal stability and retained its binding ability for biotin after exposure to temperatures of 42 degrees C for up to 3 hours, while the noncrosslinked antibody was inactivated at this temperature in several minutes.
