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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Molecular analysis of recombinase-mediated cassette exchange reactions catalyzed by integrase of coliphage HK022

Natalia Malchin1, Tatiana Molotsky, Ezra Yagil

  • 1Department of Biochemistry, Tel-Aviv University, Ramat-Aviv, Tel-Aviv 69978, Israel.

Research in Microbiology
|October 14, 2008
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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