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Updated: Jun 29, 2026
08:51
Quantification of Coenzyme A in Cells and Tissues
Published on: September 27, 2019
Propionate sensor using coenzyme-A transferase and acyl-CoA oxidase
Koji Sode1, Wakako Tsugawa, Minoru Aoyagi
1Department of Biotechnology, Tokyo University of Agriculture and Technology, Naka-cho, Koganei, Tokyo 184-8588 Japan. sode@cc.tuat.ac.jp
Protein and Peptide Letters
|October 16, 2008
Summary
We created a new propionate sensor using two enzymes. This biosensor accurately detects propionate concentrations between 10 and 100 micromolar, offering a sensitive tool for analysis.
Area of Science:
- Biochemistry
- Biosensor Technology
- Enzyme Engineering
Background:
- Propionate is a crucial short-chain fatty acid in biological systems.
- Accurate propionate detection is vital for various applications, including metabolic studies and industrial processes.
- Existing detection methods may lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop a novel amperometric sensor for propionate detection.
- To utilize recombinant enzymes for enhanced sensor performance.
- To establish the sensitivity and linear range of the developed propionate sensor.
Main Methods:
- Recombinant expression and purification of propionate coenzyme A CoA transferase and short-chain acyl-CoA oxidase.
- Immobilization of enzymes onto an electrode surface for amperometric detection.
- Electrochemical measurements to determine sensor response to varying propionate concentrations.
Main Results:
- The developed sensor demonstrated a linear response to propionate concentrations ranging from 10 to 100 micromolar.
- The detection limit for propionate was determined to be as low as 10 micromolar.
- The sensor performance was attributed to the synergistic action of the two employed recombinant enzymes.
Conclusions:
- A sensitive and reliable amperometric propionate sensor has been successfully developed.
- The sensor utilizes a novel combination of recombinant enzymes, offering a promising tool for propionate quantification.
- This biosensor has potential applications in fields requiring precise propionate measurement.
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