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Hepsin, a cell membrane-associated protease. Characterization, tissue distribution, and gene localization
A Tsuji1, A Torres-Rosado, T Arai
1Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109.
The Journal of Biological Chemistry
|September 5, 1991
Summary
Hepsin, a membrane-associated serine protease, is localized to chromosome 19 and found in most tissues, primarily the liver. Its cellular localization suggests a role in cell growth and function.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Hepsin (a serine protease) was initially identified as a human liver cDNA clone.
- Its precise function and cellular localization were previously undetermined.
Purpose of the Study:
- To characterize the human hepsin gene and protein.
- To determine the cellular localization and tissue distribution of hepsin.
Main Methods:
- Gene localization using chromosome mapping.
- Messenger RNA (mRNA) analysis to determine size and tissue distribution.
- Protein analysis to determine molecular weight and cellular localization via cell culture studies.
Main Results:
- The human hepsin gene is mapped to chromosome 19 (q11-13.2).
- Hepsin mRNA (1.85 kb) is widely expressed, with highest levels in the liver.
- The mature 51 kDa hepsin protein is a type II membrane protein, with its catalytic domain on the cell surface and N-terminus in the cytosol. It is not secreted into the culture medium or found in the cytosol.
Conclusions:
- Hepsin is a type II membrane-associated serine protease.
- Its expression pattern and localization suggest a significant role in cell growth and function.