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Nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations
M C Brahimi-Horn1, M L Guglielmino, A M Gaal
1Division of Wool Technology, Melbourne Laboratory, Parkville, Australia.
Analytical Biochemistry
|July 1, 1991
Abstract:
Lipolytic enzymes subjected to nondenaturing polyacrylamide gel electrophoresis were electrophoretically transferred under nondenaturing conditions onto a solid-state matrix. Electrotransfer permitted the visualization of hydrolytic activity to the long-chain water insoluble substrate alpha-naphthyl palmitate. Four commercial preparations: a lipase from Candida cylindracea, an esterase from porcine liver, a lipase from Pseudomonas sp., and a cholesterol esterase from Pseudomonas fluorescens were examined.