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Some remarks on the bias of the MPN method
1Department of Microbiology and Biotechnology, University of Horticulture and Food Industry, Budapest, Hungary.
International Journal of Food Microbiology
|June 1, 1991
Abstract:
The estimation of cell number by using the MPN method was studied mathematically for three parallel inoculations from three decimal dilutions. As a result of computation it can be established that these estimations at a range of microbial numbers over 10 are biased. Confidential estimation could be obtained at cell numbers between 0 and 10 accepting only a few combinations of positive and negative test tubes. These are 1 0 0, 2 0 0, 3 0 0, 3 1 0 and 3 2 0.