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Updated: Jul 16, 2026

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles
Published on: November 30, 2022
Steroid determinations in human ovarian follicular fluid using reversed phase liquid chromatography
E Vanluchene1, P De Sutter, M Dhont
1Department of Gynaecology, State University of Ghent, Belgium.
Abstract:
A method is presented, based on high performance liquid chromatography (HPLC) with u.v. absorbance detection, to simultaneously analyse all major unconjugated steroids in ovarian follicular fluids. The total analysis time is only 30 min. The use of a 3 mm i.d. column allows us to obtain detection limits for 3-oxo-4-ene steroids of 2 ng/ml. Calibration curves are linear in the 10-20,000 ng range per injection. Excellent agreement is obtained with the results using a previously published gaschromatography method.
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