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Celiac trunk cannulation for obtaining abomasal lymph from cattle.
D G Baker1, L J Gershwin, T G Snider
1Department of Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
American Journal of Veterinary Research
|July 1, 1991
Summary
Celiac trunk cannulation in cattle provides a valuable surgical method for studying immune responses to gastrointestinal parasites like Ostertagia ostertagi at the local lymph level.
Area of Science:
- Veterinary Surgery
- Immunology
- Parasitology
Background:
- Gastrointestinal parasites, particularly Ostertagia ostertagi, significantly impact cattle health.
- Understanding local immune and physiological responses in cattle is crucial for effective parasite management.
Purpose of the Study:
- To evaluate the efficacy of celiac trunk cannulation in Holstein steers.
- To assess the feasibility of using cannulated steers for immunological and biochemical analyses related to parasitic infections.
Main Methods:
- Surgical cannulation of the celiac trunk was performed in 26 Holstein steers.
- Lymph was collected for immunologic and biochemical assays.
- Lymph flow rate and white blood cell (WBC) counts were monitored post-surgery.
Main Results:
- Successful celiac trunk cannulation was achieved in 88.5% of steers.
- Lymph collection was feasible for up to 7 days post-surgery in over half of the successfully cannulated animals.
- WBC counts and lymph flow provided physiological data in infected and non-infected steers.
Conclusions:
- Celiac trunk cannulation is a viable surgical technique for cattle research.
- This method facilitates the study of local immune and physiological responses to Ostertagia ostertagi infection.
- The technique supports investigations into cattle parasitic diseases.